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High-end disinfection manufacturer
NAI-XDY-P type endoscope microbiological tester is used together with endoscope detection sampler. Use the endoscope detection sampling pump NAI-XDY-PQ for sampling. After the on-site sampling is completed, the cup is closed and then transferred to the microbiology laboratory. The NAI-XDY-P type microbiological tester is used for suction filtration to keep microorganisms on the membrane , Count after incubation. Suitable for endoscopic microbial load detection in the medical and health industry.
Manufacturer of endoscope microbiological detector
1. Built-in miniature high-performance diaphragm pump, no need to filter the bottle, greatly reducing the occupation of the operating space and more convenient use;
2. Push button switch control with indicator light, simple and intuitive operation;
3.Simple internal pipelines, no dead corners for breeding of microorganisms, easy to clean and disinfect;
4.Stainless steel cabinet is polished by mirror, the surface is smooth and flat, easy to clean and disinfect;
Manufacturer of endoscope microbiological detector
1. Applicable consumables: endoscope inspection sampler
2. Power supply: AC 220V / 50Hz
3. Power: 25W
4. Pump flow: 0.7L / min
5. Noise: ≤60dB (load state)
6. Weight: 2.5kg
7. Dimensions: 25cm × 19cm × 14cm (length × width × height)
8. Drain hose specifications: inner diameter Φ10mm ～ Φ16mm silicone tube
Endoscopy sampling and microbial limit testing in the healthcare industry
Because of its unique structural characteristics, endoscopes are difficult to clean and disinfect, and there is a hidden danger of cross-infection in patients. Therefore, it is particularly important to regularly monitor the effectiveness of endoscope cleaning and disinfection to evaluate whether the disinfection meets the standards. The 2015 edition of the Chinese Pharmacopoeia requires the use of a membrane filtration method to check the microbial limits of purified water. This method is also mentioned in the monitoring of endoscopes after disinfection in the "Sanitary Standards for Hospital Disinfection" (GB 15982-2012). 50mL eluent The amount can more fully contact the inner surface of the endoscope than 10mL. Combining the direct inoculation method, membrane filtration method and colony count will more objectively and scientifically evaluate the quality of endoscope disinfection.
The "Specifications for Cleaning and Disinfection of Soft Endoscopes" (WS507-2016) requires biological monitoring of disinfected endoscopes on a quarterly basis. The sampling method for endoscopes after disinfection described in "Sanitary Standards for Hospital Disinfection" (GB 15982-2012) is membrane filtration Method: Take the endoscope after cleaning and disinfection, use a sterile syringe to extract 50 mL of the eluent containing the corresponding neutralizing agent, inject the flushing endoscope tube from the biopsy port, and collect the entire amount (peristaltic pump can be used) for inspection. Mix the eluent thoroughly, inoculate 1.0 mL of the eluate and inoculate the dish, pour 15 mL-20 mL of molten nutrient agar medium cooled to 40 ℃ ~ 45 ℃, incubate in a 36 ℃ ± 1 ℃ incubator for 48h, count the colonies Number (CFU / piece). The remaining eluate was filtered and concentrated under a sterile condition using a filter (0.45 μm). The filter was inoculated on a solidified nutrient agar plate (be careful not to generate air bubbles). Incubate at 36 ° C ± 1 ° C for 48h. Count the colonies. number.
When the membrane method cannot be counted: the total number of colonies (CFU / piece) = m (CFU / plate) × 50 where: m is the average colony number of two parallel plates.
When the filter method can be counted: the total number of colonies (CFU / piece) = m (CFU / plate) + mf (CFU / filter) Where: m is the average number of colonies on two parallel plates; mf is the number of colonies on the filter.
The membrane sampling method involves instrument materials used in endoscopic sampling including a bacterial filter or a microbial limit detector (with a filter) and a 0.45 μm filter.
Bacterial filter: It consists of vacuum pump system, collection bottle, connection tube, filter rack, filter cup, flame sterilizer, etc.
Microbial limit detector: It consists of a filter drainage box, a flame sterilizer, and a filter cup.
Filter cup (filter): There are commercially available filter cups made of stainless steel and modified PP, both of which can be sterilized by damp heat and flame gun.
Filter membrane: "Chinese Pharmacopoeia" stipulates that the pore diameter of the filter membrane used for sterility inspection should not be greater than 0.45μm and the diameter is about 50mm. The filtration capacity of 0.45μm filter membrane can reach more than 99.99%, and it has less damage to microorganisms than 0.22μm filter membrane, which is more conducive to the recovery and cultivation of microorganisms. Commercially available 0.45 μm filter membranes come in two diameters of 47 mm and 50 mm. Note that the filter should be sterilized before use.
The vacuum pump vacuum filtration principle is used to create a pressure difference between the top and bottom of the microporous membrane (sterilized before use) in the filter cup. The test product in the filter cup passes through the microporous membrane due to the pressure difference. The microporous filter membrane has a complex honeycomb structure, and even microorganisms below 0.45 μm can be intercepted, thereby efficiently recovering microorganisms and trapping the microorganisms that may be present in the test product on the filter membrane. The pore size of the 0.45 μm filter membrane is beneficial to the recovery of microorganisms, and the culture medium is easily permeable to the filter membrane, which is beneficial to the cultivation of bacteria.
Filter method operation steps:
1. Take out the sterilized filter cup and connect it to the host to ensure that the seal is intact;
2. Inject the remaining eluate after the direct inoculation method into the filter cup, and vacuum filter through the vacuum pump. The eluent in the filter cup passes the microporous membrane (sterilized before use) due to the pressure difference. Microorganisms that may be present in the dehydration are trapped on the filter membrane;
3. Remove the filter cup, remove the filter membrane with sterile forceps, and transfer to the configured solid medium with the bacteria side facing up and flat. The filter membrane should be completely attached to the medium without air bubbles (bubbles) Will affect the growth of microorganisms);